Muhammad Mushtaq
E-mail | [email protected]
... Further trials with low concentrations of strychnine alkaloid and decreasing the poison-baiting duration are suggested.
...
Gultaj Hussain1, Abdur Rahman1*, Tariq Hussain2, Siraj Uddin1, Tariq Ali3
E-mail | [email protected]
...f citric and lactic acid concentrations effect were maximum for 5.0%, followed by 3.0% and least for 1.0% on E. coli and Salmonella load on beef surfaces. In addition, citric acid was more effective than lactic acid at all concentrations (1.0, 3.0 and 5.0%) in reducing E. coli and Salmonella growth. The citric acid at 5.0% was found significantly effective treatment in reducing E. coli O157: H7 and S. typhymurium population ...
Mudasir Irfan Dar*, Fareed Ahmad Khan and Farha Rehman
E-mail | [email protected]
...ere exposed to different concentrations of the insecticide, ranging from 0 to 40 grams active ingredient per hectare (g.a.i ha-1) through foliar spray. Analyses were done at days 3, 7, and 15 after treatment. Lipid peroxidation rates and contents of proline, ascorbate (ASC), glutathione (GLU), antioxidative enzymes; superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) were assessed. Imidacloprid enhanced malondi...
Ghulam Ali Bajwa1, Faizah Abood2, Yusof Bin Ibrahim3
...ays for different tested concentrations. A significant (p<0.05) 2nd degree correlation between temperature and B. bassiana infectivity was analysed. Beauveria bassiana infection was in range of 27-30ºC. Based on these findings, it is concluded that B. bassiana has promising potential as microbial control agent against A. sciodoxa and should be exploited under field conditions....
John G. Bruno1*, Chien-Chung Chao2,3, Zhiwen Zhang3, Wei-Mei Ching2,3, Taylor Phillips1, Allison Edge1, Jeffery C. Sivils1
...ss-react with comparable concentrations of E. coli or L929 murine host cells, the assay did cross-react strongly with members of the spotted fever and scrub typhus groups, making the assay specific only to the Rickettsiaceae family level (Rickettsia and Orientia). When conducted in < 100% human serum or a tick homogenate, the fluorescent aptamer assay maintained sensitive detection of Rickettsia typhi
Nazish Huma Khan*, Mohammad Nafees and Adila Bashir
... showed that the average concentrations of metals in soils of orchard and non-orchard fields were as chromium (Cr) 56.9 and 52.2 mg kg-1, Ni 29.8 and 27.9 mg kg-1 and Cd 2.46 and 1.11 mg kg-1, respectively. The risk contribution from Cd, Cr and Ni were significant in crop seeds of orchard field (THQ>1). The substantial load of heavy metals in studied fields showed the anthropogenic source of pollution. Therefore regular moni...
Ishfaq Ahmed, Ihsan Mabood Qazi and Suraiya Jamal
...flour (BRF) at different concentrations (0, 20, 40, 60, 80 and 100 g/100 g) on the proximate compositions and functional properties. It was found that percent protein (8.93–12.25%), ash (0.52–1.01%) and moisture contents (4.47–7.09%) showed significant (P < 0.05) increase, while fat content (1.41-1.17%) showed significant (P < 0.05) decrease by blending WF with BRF. Similarly, water solubility index (WSI), water absorption index (WAI) a...
Zafar Khan, Asad Sultan, Rajwali Khan, Sarzamin Khan, Imranullah and Kamran Farid
...Fe, Mn and Zn). The mean concentrations of toxic heavy metals; Pb, Cd and Cr in albumen samples collected from Peshawar were 0.13, 0.06 and 0.09 (ppm) respectively. Concentrations of Pb, Cd and Cr in egg albumen of Dir Lower were 0.13, 0.05 and 0.05and in Malakand were 0.12, 0.05 and 0.07 ppm, respectively. The mean concentrations of essential elements Fe, Mn and Zn in egg yolk of Peshawar...
Muhammad Zahid1, Muhammad Hamid Bashir1*, Bilal Saeed Khan1 and Muhammad Shahid2
... N. barkeri at different concentrations under controlled laboratory conditions. The results showed 25%, 30%, 70%, 50% and 65% mortality occurred by tested pesticides at field relevant doses after 144 h respectively. Missing mites data indicated repellency due to pesticides, was highest at the start of experiment, and then these acclimatized on leaf discs. Thiacloprid having lowest LC50 value 295 and proved toxic for the predator while diafenthiuron and pyripro...
Ayanda Opeyemi Isaac,1,* Oniye Sonnie Joshua2 and Auta Jehu2
...exposed to varying acute concentrations of glyphosate and paraquat. The fishes responded, exhibiting different behavioural abnormalities like hyperactivity, abnormal swimming, restlessness, loss of equilibrum and haemorrhage. Observation of opercular ventilation count (OVC), tail fin movemant rate (TMR) and air gulping index (AGI) showed a marked difference between control and exposed fishes, indicating that the herbicides negatively impact on these parameters...
Misbah Riaz1, Qaiser Mansoor2, Maleeha Akram1, Muhammad Ismail2, Parveen Akhtar3, Shakeel Mirza4, Mazhar Qayyum1, Afzaal Ahmed Naseem1, Faheem Tahir5 and Syed Shakeel Raza Rizvi1*
...typic traits such as low concentrations of FSH, LH and T and delayed puberty. In conclusion, mutations in GNRHR may cause delay in male puberty in Pakistani population....
Afzaal Ahmad Naseem1*, Maleeha Akram1, Sarwat Jahan2, Kiran Afshan2, Zubaria Iqbal1, Faheem Tahir3, Mazhar Qayyum1 and Syed Shakeel Raza Rizvi1
...=27 boys/age group). The concentrations of GH and T were measured using specific ELISA systems and LGV was calculated for each age/stage group. Pearson correlation r and ANOVA were employed. GH and LGV exhibited age related positive correlations from 1 to 20 years and developmental stage dependent positive correlations during infancy, pre-puberty, early, mid and late puberty/adolescence. T and LGV were positively correlated from 1st to 16th year and negatively...
Taghreed A. Hafiz1, Murad A. Mubaraki1*, Mohamed A. Dkhil2,3 and Saleh Al-Quraishy2
...od, respectively. Serial concentrations of the extract not exceed the maximum nontoxic concentration were used against Vero cells for anti-HSV-1 and ant-HSV-2 effects by plaque assay. The results of the phytochemical analysis showed that the extract has high polyphenolic content while has relative low flavonoids contents. In the antiradical scavenging property test the extract showed at 64.1 μg/ml 50% DPPH radical scavenging activity and in the MTT assay th...
Shakeel Ahmed* and Sarwat Jahan
...s. The plasma adipokines concentrations were determined by using specific Enzyme Immunoassay (EIA) kits. 48 h fasting significantly increased plasma adiponectin (P<0.001), while decreased leptin (P<0.001) and resistin (P<0.01) concentrations compared to normal fed monkeys. No significant (P>0.05) change in adiponectin levels was observed after NMS injection in both normal and metabolically stressed conditions. NM...
Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2
...ine oocytes at different concentrations. The results showed that the blastocyst rates in injection 560 ng/μL Npm and 1000 ng/μL PGA groups were higher than those of other groups. Moreover, the blastocyst rate was highest in injection 560 ng/μL Npm group and was lowest in injection 100 ng/μL PGA group. These data suggested that addition of recombinant Npm obtained by prokaryotic expression or PGA could improve the development of SCNT embryos in the ...
Faiqah Ramzan*1 and Muhammad Haris Ramzan2
...ally in different dosage concentrations (1μg, 1 ng, and 10 ρg) to adult male mice twice daily for 12 days. Prostatic citric acid levels were determined photometrically. The levels decreased significantly at all tested doses. Intermittent administration of Kisspeptin –10 negatively regulates the prostatic citric acid levels in adult male mice....
Dilawar Hussain* and Abdul Mateen
...at both 2 and 4 ppm AFB1 concentrations have almost the same effect on the growth parameters tested except in the case of PER. T3 (0.5% 4TX+2 ppm AFB1) showed better PER (31.42±1.74) when compared to T4 (0.5% 4TX+ 4 ppm AFB1) group (27.76±0.67). Interaction between different growth parameters of tilapia fed AFB1 and 4TX supplemented diets showed that net weight gain (NWG) was significantly (p<0.01) correlated with average daily gain (ADG), spe...
Wang Tian1, Huayong Zhang1,*, Jian Zhang2, Lei Zhao1, Mingsheng Miao3 and Hai Huang1
...ich provides the maximum concentrations of nutrients. Zooplankton communities were more correlated to phytoplankton community biomass than environmental factors. Results of canonical correspondence analysis (CCA) revealed that water temperature, Secchi disk depth (SD), total phosphorus and phytoplankton biomass were the most significant factors that influenced zooplankton. Strong correlations between SD and Brachionus leydigi, Chrysophyta and Diaphanosoma leuc...
Sidra-Tul-Muntaha1, Muhammad Sagheer1*, Mansoor-ul-Hasan1 and Shahbaz Talib Sahi2
... Punjab, Pakistan. Three concentrations of each plant extract (5, 10, 15 and 20%) and synthetic pyrethroids (0.01, 0.02 and 0.03%) were evaluated in this study. We observed significant results with each treatment. For both repellent and growth inhibitory effects, A. indica and deltamethrin were most efficient among plant extracts and pyrethroids respectively. At highest dose rates, more than 90% repellency was recorded with both A. indica and deltamethrin. Upt...
Salma Shaheen, Mumtaz Khan, Muhammad Jamil Khan, Saleem Jilani, Zarina Bibi, Muhammad Munir and Mehwish Kiran
...M. Maximum Fe, Cu and Mn concentrations were found where pressmud + EM was applied. Dietary fiber, vitamin C and crude proteins were also increased in spinach with pressmud + EM application. It was concluded that EM-inoculated pressmud has higher potential to increases soil fertility as well as stimulate spinach growth and quality. Therefore, warrants further testing under field conditions....
Blood Selenium and Vitamin E Levels in Heifers: Regional and Seasonal Differences in Northern Cyprus
Serkan Sayiner1* and Hilal Karagul2
... evaluated whether these concentrations were adequate. Heifer blood, feed samples including concentrate feed or roughage were analysed. It was found that there are significant seasonal differences for each region and regional differences in each season were due to different feeding conditions. Heifer blood selenium and plasma vitamin E levels were generally found within adequate ranges in all regions for both seasons. We suggest that feed supplements containin...
Sumra Ashraf1, Zain ul Abdin1,*, Saqi Kosar Abbas2,Rao Sohail Ahmad Khan3, Muhammad Tahir1, Sehrish Rasool1, Maryam Anwar1 and Fiaz Hussain1
Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
... chickens with different concentrations of plasmid DNA and the sera were collected weekly post vaccination for antibody detection by HI test. All immunized chickens shown high HI antibody titers (9Log2) two weeks post-booster dose. The chickens were then challenged using homogenous AI H9N2 strain. During the course of infections, oropharyngeal and cloacal swabs were collected from all chickens at 3, 5 and 7 days post-challenge (p.c.) for virus titration in egg...
Adel Eid Mohamed Mahmoud*
...ues and ammonia nitrogen concentrations did not show any significant differences among groups.No significant differences were observed in meat chemical analysis of slaughtered animals among different groups. Animals fed BBP recorded insignificant increase in growth rate by 9 g daily with improvement in feeding cost by 10%.Bakery waste can replace corn grains and wheat bran in concentrate feed mixtures up to 60 % in growing lambs rations without any adverse eff...
A. K.Bhattacherjee and B. K. Pandey
Dissipation of carbendazim in mango after pre- and post-harvest treatments
...at 0.05 and 0.1 per cent concentrations, respectively, following first order rate kinetics. The corresponding values in fruit pulp after 12 days were 0.57 and 1.26 mg kg . In another experiment, cold water dip treatment in carbendazim solution @0.05 and 0.1 percent was given for 10 min to a separate lot of fruit having no pre harvest spray. Dissipation of carbendazim in whole fruit again followed first order rate kinetics with 0.92 and 2.06 mg kg of the fungic...
M.S. Islam, M.Ali, and I. Ahmad
Efficacy of sedomil 72WP and recozeb 80WP to control die-back (Colletotrichum gloeosporioides) of tea in Bangladesh
... 1200, 1600 and 2000 ppm concentrations. Sedomil 72WP and recozeb 80WP caused colony growth inhibition, respectively by 75.00-90.77% and 75.88-91.33% in 2008 and 74.66-90.55% and 75.66-91.11% in 2009. In a field experiment at the main farm of Bangladesh Tea Research Institute (BTRI), both fungicides were applied as foliar spray for three times at 1.0, 1.5, 2.0 and 2.5 kg ha-1. In 2008, sedomil 72WP gave 45.52-92.78% and recozeb 80WP gave 46.47- 90.39% reductio...
Anil Sehajpal, Saroj Arora and Parminder Kaur
Evaluation of plant extracts against Rhizoctonia solani causing sheath blight of rice
...bial effect at different concentrations against the pathogen whereas 8 plant extracts, viz. Abrus precatorious, Acacia auriculiformis, Bougainvillea glabra, Convolvulus arvensis, Hibiscus rosa-sinensis, Morus alba, Thevatia peruviana, and Withania somnifera did not exert any effect. Among all the plant extracts, A. sativum exhibited strong fungitoxicity even at the lowest concentration, i.e. 100 ppm, with relative magnitude of inhibition 2.0 mm against the pat...
Sohail Ahmed, Muhammad Arshad and Babar Hassan*
...ee resins types in three concentrations were applied by brushing and dipping on wooden stakes which were exposed to termites’ for a period of 4 weeks in the field. Weight loss before and after resin treatment was indicative of effectiveness. Highest concentration of all resins significantly reduced the chances of termites’ infestation as compared to other concentrations by dipping application in contrast to coati...
Burhan Azam1, Muhammad Naeem Tahir2,*, Faisal Shahzad2, Abdul Ghaffar3, Ghulam Abbas4, Madiha Gohar5 and Saima1
...lids not fat and lactose concentrations differed (P<0.001) among the treatments but did not show (P>0.05) any linear or quadratic trends with increasing level of enzyme in the diet. Milk protein concentration linearly (P<0.001) increased with increasing level of enzyme in the diets. It is concluded that fibrolytic enzyme addition in the diets of lactating Nili Ravi buffaloes improves the dry matter intake and diet digestibility which results in improv...
Obaidullah1, Zafar Ali Shah1* and Sami Ullah2
...;g/ml and 80 µg/ml concentrations versus their counterparts of Lapachol and above mentioned metallic salts....
Viram Kumar1,*, Moolchand Malhi1, Saeed Ahmed Soomro1, Toufique Ahmed Qureshi2, Muhammad Nawaz Sanjrani3 and Khushal Das Malhi1
...ed that RBC, MCV and MCH concentrations were significantly higher (P < 0.05) and the serum total protein and Mg concentrations were significantly lower (P < 0.05) in male compared to female peafowl. In conclusion, the present study provides base-line values for hematology and serum biochemistry of apparently healthy Indian adult blue peafowl of Thar and can be used as reference values to evaluate the health or diseased...
Muzammil Sattar
...ed in laboratory at four concentrations, i.e., 1, 3, 5 and 7 mg/ml in diet. Significantly higher fecundity 785.12±25.7, fertility 89.23±0.36 % of eggs and longer oviposition period 42.13±1.11 days were recorded in adults feeding on a diet containing Nu lure followed by protein hydrolysate (control diet). The F1 progeny larvae that developed from adults consuming (Nu lure 5ml) diet had the shortest larval and pupal periods...
Mehwish Faheem1,*, Saba Khaliq2 and Khalid Parvez Lone2
...i> was exposed to graded concentrations of bisphenol-A (10,100,1000μg/l) for 14 days. BPA exposure strongly elevates vtg mRNA level in fish exposed to 100μg/l but at 1000 μg/l exposure of BPA, vtg level decreased compared to 100µg/l exposed fish. These results showed that BPA has estrogenic action and cause endocrine disruption in juvenile C. catla at environmentally relevant concentration. Moreover, these results also depic...
Hazrat Ali1,*, Wajid Ali1, Karim Ullah1, Fazle Akbar1, Sher Ahrar1, Irfan Ullah1, Irum Ahmad1, Ashfaq Ahmad1, Ikram Ilahi2 and Muhammad Anwar Sajad3
...arison of both Cu and Zn concentrations in muscles of the study fish generally showed no significant differences among different sites on the rivers. However, in some cases, metal concentrations showed significant increase down the stream. Similarly, a comparison of metal concentrations in muscles of the two species generally did not show significant differences between the two species. Zn...
Noor Islam1, Muhammad Amjad2, Ehsan ul Haq3, Elizabeth Stephen1 and Falak Naz4
... acid at three different concentrations of 25%, 50% and 100% and control were used against T. clareae in naturally infested honey bee, A. mellifera colonies in Honeybee Research Institute, National Agricultural Research Centre, Islamabad, Pakistan. The percentage of infestation by T. clareae on worker brood, number of dead/fallen mites per hive/weekon white formica sheet, percent mite mortality and honey yield per colony was determined bef...
Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...ckens with two different concentrations of Egy-H5 plasmid DNA 15µg and 60 µg. Results demonstrate that chickens developed detectable HI antibody titers up to 6log2 within two weeks post-booster vaccination. The challenge experiment was performed using isolate A/chicken/Egypt/A6/2011 (H5N1) with concentration of 105 EID50 to evaluate the protective efficacy of the Egy-H5 plasmid DNA vaccine. The Egy-H5 plasmid DNA vaccine induced complete protection...
Sumaira Maqsood1,*, Muhammad Afzal1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1,Waqas Wakil2 andMuhammad Hussnain Babar3
...ved more fatal at higher concentrations than the individual application at lower concentration for pupation and adult emergence of S. litura. Apart from untreated larvae (control) maximum pupation (66.01±1.32 and 73.95±1.28 %) and adult emergence (61.87±1.71 and 67.77±1.11 %) was observed when larvae were treated with NPV (1x107 POB/ml) against 2nd and 4th instar larvae. This study would be he...
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
...r than lower (P<0.05) concentrations. However, double stranded DNA was stained with lower concentration of dye in staining solution. In conclusion, the buffalo sperm should be stained with higher concentration of AO stain in washed sample either with NS or PBS and heated incubation in tampon solution for better assessment of fragmented DNA.
...
Syed Tanveer Shah* and Muhammad Sajid
...lphate) at three calcium concentrations (0.5, 0.75 and 1.0%) stored for 30 days at 10 days interval. The experimental results showed that calcium sources, concentration and storage duration significantly affected all the studied attributes. More fruit firmness (5.57 and 5.69 kg cm2), less Total soluble solids [TSS] (8.57 and 8.26 °Brix) and TSS-acid ratio (12.62 and 11.48) were recorded by the alone application of calcium chloride at 1.0% calcium concentra...
Fang Wang, Yong-Fang Jia, Po Wang, Qi-Yan Du and Zhong-Jie Chang*
...ere exposed to different concentrations of LET. At 130 dph, sex ratio was recorded. The results showed that continuous exposure to LET induced the changes of sex ratio. High concentration of LET can promote male-biased sex ratio. In order to reveal the molecular mechanism by which LET caused sex reversal, juvenile carp (120dph) were exposed to 0, 5, 25, 125, and 625μg/L LET for 15 days, and the expression profiles of seven gonad differentiation related gene...
Zeenat Bano1,*, Sajid Abdullah1, Waqas Ahmad2, Muhammad Anjum Zia3 and Wardah Hassan1
... from all the sites. The concentrations of Zinc (Zn), copper (Cu) and nickel (Ni) were higher in river water as compared to farm and hatchery. Different organs of fish collected from different sites showed variations in their metal concentration. In Indus River fish, the concentrations of metals were highest in liver followed by kidney and gills. The Indus River fish also exhibited highest SOD activity in all the orga...
Bibi Haleema1*, Abdur Rab1 and Syed Asghar Hussain2
...mparing the means for Zn concentrations, maximum plant height (86.53 cm), number of primary (2.53) and secondary (6.42) branches, leaves plant-1 (167), leaf area (63.33 cm2), and fruit per plant (63.78) were higher with 0.5% foliar Zn application. The interaction between Ca, B and Zn also showed significant results for most of the attributes. Therefore, application of Ca (0.6%), B (0.25%), and Zn (0.5%) as a foliar spray can be used alone or in combination to ...
Faiza Ambreen*1 and Muhammad Javed2
...and then four sub-lethal concentrations viz. 1/3rd of LC50, 1/4th of LC50, 1/5th of LC50 and 1/6th of LC50 were calculated and fish were exposed to these concentrations, separately in glass aquaria for 70 days at constant laboratory conditions. Peripheral blood erythrocytes were taken on 14, 28, 42, 56 and 70 day of exposure for th...
Naila Riaz1 , Hafiz Muhammad Tahir2* , Khizar Samiullah3
...topically with different concentrations of the crude venom. All aphids died within 6 hr with oral treatment at 1.5µl of the venom dose. However, at the same venom dose, 100% mortality was achieved at 8 hr posttreatment when applied topically. Similarly, oral administration of 1µl and 0.5µl venom caused 100% mortality at 8 hr post-treatment compared to 10 hr and 12 hr post-treatment of topical application, respectively. It is concluded that or...
Irfan1* , Arshad Javid2 , Muhammad Altaf3 , Muhammad Shahbaz3 , and Khalid Javed Iqbal4
...ignificantly higher urea concentrations 33.45±3.39 mg/dL were recorded from the serum of the birds reared under free range rearing system. However, non-significant variations in albumin, total protein, ALP and AST were recorded for all the three rearing systems. Significantly higher values of cholesterol, globulin, albumin, creatinine and ALT were recorded from serum of male M. gallopavo while urea and ALP were higher in hens’ serum. However, non-...
Ayesha Aihetasham1*, Khalid Zamir Rasib2, Syeda Rida Hasan1, Imran Bodlah3
...al efficacy of different concentrations of aqueous leaf extracts of three medicinal plants viz., Carica papaya (paw paw), Helianthus annus (Sunflower) and Bougainvillea glabra (Paper flower) against Heterotermes indicola. The leaf extract of C. papaya caused highest mortality i.e. 100% of 10%, 5% and 3% concentration. Bougainvillea glabra and H. annus caused 100% mortality at 10% and 5% concentration while 96.4% mortality on 3% concentration after exposure per...
Maryum Fakhar, Iqra Jabbar, Anjum Nasim Sabri*
... presence of nickel. Two concentrations of nickel were used i.e., 500μg/ml and 1000μg/ml for subsequent tests. The results revealed that at low/ sub-inhibitory concentration of nickel (500 μg/ml), motility is inhibited; adhesion, hydrophobicity, aggregation, EPS production and biofilm formation of two strains are increased. This biofilm forming ability can be employed in
urification of waste waters and treating contaminated soils....
urification of waste waters and treating contaminated soils....
Rimsha Nawaz1, Muhammad Javed1, Sidra Abbas1* and Safina Kousar2
.../sup> of LC50 concentrations of iron chloride for 30 days in the glass aquaria with three replications for each treatment. After 30-day exposure of iron chloride, the fish organs viz. kidney and liver were isolated and the peroxidase enzyme activity was analyzed. Activity of peroxidase was assessed by measuring the conversion of guaiacol to tetraguaiacol, spectrophotometrically, at a wavelength of 470 nm. The peroxidase activity was measured ...
Sumaira Zareen1, Syeda Sadaf Zahra2, Ayeza Mehmood3, Muhammad Asadullah4* and Aish Muhammad5
...plemented with different concentrations and combinations of benzyl amino purine (BAP) or kinetin for shoot induction and Indole acetic acid (IAA) for primary shoot and Indole Butyric acid (IBA) for root proliferation. Best shoot proliferation (4.48 per explant) was observed in MS medium containing 0.2/0.4 mg/L BAP/IAA combination. For rooting of the micro shoots, MS medium was supplemented with 1.0 mg/l IBA exhibited best results with 4.69 mean value of roots ...
Muhammad Naeem1, Bushra Sadia1,2,*, Faisal Saeed Awan1and Muhammad Anjum Zia3
...th ammunium sulphate, at concentrations as low as 30 μl. Optimized conditions for maximum yield of mutant SK included CSL 6%, 24 h. of fermentation, pH 7.5, 37oC, KH2PO4 0.04%, K2HPO4, NaHCO3 0.15% and 0.015% CH3COONa. The results indicated that UV is the most effective mutagen for hyper-production of SK. Mutant strain UV6 has the potential for enhanced SK production with commercia...
Ata-ul-Mohsin*, Ehsan-ul-Haq** and Muhammad Naeemullah*
...cium and high phosphorus concentrations applied to a partially resistant wheat cultivar “Rapier” did not have a significant effect on the growth and development of the aphid or its parasitoid. However, the mean fecundity of the parasitoid was higher on plants with high phosphorus than those treated with high calcium concentrations....
Jaime Molina-Ochoa1, 2, 6,*, Edelmira Galindo-Velasco2, Ana María Rosales-Gutiérrez2, Martín González-Ramírez3, Roberto Lezama-Gutiérrez3, Wilberth Chan-Cupul3, Steven R. Skoda4, Muhammad Irfan Ullah5, Luis Jorge García-Márquez2 and John E. Foster6
Muhammad Arshadullah, Syed Ishtiaq Hyder, Arshad Ali and Imdad Ali Mahmood*
CUMULATIVE EFFECT OF SULFUR AND CALCIUM ON WHEAT GROWTH AND YIELD UNDER SALINE-SODIC SOILS
...um (K), Na, Ca, S and Mg concentrations in grain were estimated using atomic absorption spectroscopy. Tillering, -1 grains spike , 1000-grain weight and paddy yield significantly (P≤0.05) enhanced by increasing the rate of gypsum (CaSO ). The maximum 4 -1 number of grains spike (60), 1000-grain weight (47 g) and grain yield -1 -1 (4.01 t ha ) were recorded with CaSO application @ 150 kg ha . Grain 4 yield was 43% more than control treatment. Positive correl...
Nazakat Nawaz*, Malik Shah Nawaz*, Mubashir Ahmad Khan*, Mirza M. Yasin*, Doulat Baig*, Nasir Mehmood Cheema**, Muhammad Amjad** and M. Altaf Sher**
EFFECT OF BORON ON PEANUT GENOTYPES UNDER RAINFED CONDITIONS
...equirements and critical concentrations in diagnostic parts of three peanut genotypes, BARD-479, ICG-7326 and ICGV-92023. These genotypes were grown in boron deficient soil at National Agricultural Research -1 Centre, Islamabad, Pakistan. Five rates 0.0, 0.5, 1.0, 2.0 and 4.0 kg B ha as boric acid (H BO ) were applied. Boron fertilizer applications significantly 3 3 increased dry pod yield of all the tested genotypes over control. Dry pod yields of BARD-479, I...
Riaz Hussain*, Muhammad Riaz**, Mushtaq A. Saleem*** and Muhammad Ishaque Mastoi**
BIOCHEMICAL ABNORMALITIES INDUCED BY ABAMECTIN IN SIXTH INSTAR LARVAE OF THE RED FLOUR BEETLE, TRIBOLIUM CASTANEUM (HERBST)
...etween strains and among concentrations. Abamectin, at LC and LC , changed the activities 10 20 /levels of TE, CE, AcP, total protein and FAA in the larvae of both the strains. The activities of alpha-amylase, glucoamylase and AkP remained non-significant at both doses in the two strains. In PAK strain larvae, the TE activity was inhibited with depletion of total protein contents and elevation of FAA contents. In FSS-II larvae, the effect of abamectin on level...
Muhammad Mudasar Aslam ⃰, Muhammad Jamil**, Ijaz Malook**, Amana Khatoon*, Ali Rehman*, Abdur Rahim***, Pirzada Khan*, Shakir Ullah Khan Shakir*, Shahid Irfan*, Faizan Ullah****, Khair Ul Bashar**, Mahideen Afridi** and Shafiq Ur Rehman*
PHYTOTOXIC EFFECTS OF CALOTROPIS PROCERA, TAMARIX APHYLLA AND PEGANUM HARMALA ON PLANT GROWTH OF WHEAT AND MUSTARD
... aqueous extracts at all concentrations (5%, 10%, 15%, 20% and 25%) but shoots were inhibited by higher concentrations (20% and 25%). This study revealed that wheat is more sensitive to different plant extracts as compared to mustard. It is thus concluded that inhibitory effect increases with the increase of extracts concentration....
Abdul Rehman Khan*, Muhammad Mahmood–Ul-Hassan**, Rizwan Ahmad** and Anjum Munir ***
ARSENIC III TOLERANCE POTENTIAL OF FUNGI ISOLATED FROM POLLUTED AND NON-POLLUTED SOILS OF PAKISTAN
...m amended with different concentrations of As (50 to 5600 mg/L). The fungal tolerance was evaluated by comparing tolerance index (TI), minimum inhibitory concentrations (MIC) and growth rate. Out of 18 isolates, 12 belonged to genus , 3 to , 2 to and one to . The isolates and appeared to be most tolerant to arsenic. Fungal strains isolated from Gujranwala soil exhibited more As(III) tolerance than those from Multan and Islam...
Mahmoud M. Al-Azzazy1,2 and Saleh S. Alhewairini1,*
...pared predatory mites at concentrations which had a high lethality to tomato russet mites. Hence, Huwa-San TR50 can be utilized in the Integrated Pest Management (IPM) program as an acaricide agent.
...
Filiz Kutluyer*
...tative changes different concentrations of L-tryptophan. In addition, L-tryptophan can be used in activation medium for O. mykiss, S. rizeensis and S. coruhensis.
...
Asmatullah1, Chaman Ara1,*, Shagufta Andleeb2, Maha Tahir1, Beenish Zahid1 and Madeeha Arshad1
...rpose, different aqueous concentrations of cadmium chloride i.e. 0.00, 6.25, 12.50, 25.00 µg/g B.W of mice were given orally to pregnant mice on days 6-12 of gestation daily. Another series of mice were treated with different concentrations of cadmium chloride and also with an antidote (guava fruit extract) on days 6-18 of gestation daily once a day. Fetuses were recovered on day 18 of gestation, analyzed based ...
Hao Zhang,1,2,3,* Hai Tao Nie,3 Tie Wei Ma,3 Zi Yu Wang3 and Feng Wang3
... to estimate the mineral concentrations and distributions in the main body tissues and the net requirements for maintenance and growth of F1Dorper × Hu ewe lambs. Thirty-five ewe lambs averaging 33.52±0.56 kg body weight (BW) were used. Seven ewe lambs were randomly chosen and slaughtered at 34.93 ± 0.37 kg BW as the baseline group for measuring initial body composition. Another seven lambs were also randomly chosen and offered a...
Hongwei Liang1,2,*, Miaomiao Tong1, Lihuan Cao1,2, Xiang Li3, Zhong Li1 and Guiwei Zou1,*
...red. Calipash had higher concentrations of flavor amino acids than muscle. Muscle tissue of the Huaihe River strain had the highest concentration of pharmacodynamic amino acids and the highest concentration (P < 0.05) of arachidonic acid (females: 109.83 mg/ 100g, males: 110.72 mg/100 g). Concentrations of eicosapentaenoic acid and docosahexaenoic were higher in male muscle than in female muscle. Opposite results w...
Shireen Ihsan Izzaddeen1 and Ali Kaygısız2,*
...albumin, GH and globulin concentrations did not differ between the groups (P>0.05). Essential laurel oil containing whole milk, on the other hand, lowered serum triglyceride concentration (P<0.05). Altogether these results suggested that the consumption of laurel essential oil (600 mg/day) containing whole milk by calves might have positive influences on their health parameters compared to the control group.
...
Nilgün Paksoy1, Hikmet Dinç2 and Serap Kılıç Altun3,*
...udy was to determine the concentrations of Na, Mg, P, K, Ca, Fe, Cu, Zn, Ni, Cd, V and Ba in donkey, goat, and sheep milk samples. Fifty-six individual milk samples were collected from 17 lactating donkeys, 19 goats, and 20 sheeps in three different Turkish Farms. The samples were analyzed by inductively coupled plasma - mass spectrometry (ICP-MS). Minimum and maximum levels of Na, Mg, P, K, Ca, Fe, Cu, Zn in donkey milk samples were 108.6-202.7, 48.6-76, 355-...
Ikramullah Khan1,2*, Muhammad Subhan Qureshi2, Sohail Akhtar2, Ijaz Ali3 and Ghufran Ullah4
...ose, cortisol and HSP-70 concentrations wasmaximum in HF (43.44, 119.06 and72.11%) andcrossbred (38.66, 159.61 and79.12%) and minimum inAchai (34.93, 123.59 and52.53%) and Sahiwal (27.26, 117.22 and65.41%)respectively (P< 0.01). Holstein Frisian appeared as the most heat sensitive breed exhibiting changes at 32 °C i.e. 5 °C higher than 27 °C (upper critical temperature, UCT). Sahiwal and Achai were the most heat tolerant breeds manifesting chang...
Nadia Mubarik1, Aqib Iqbal1*, Iqbal Munir1 and Muhammad Arif2
... water stress) and three concentrations of CaCl2 (5, 10 and 15 mM CaCl2.2H2O) as foliar spray on maize seedlings 20 days after emergence (DAE). Water was sprayed as untreated control on maize seedlings 20 DAE. After foliar application, the plants were regularly irrigated upto one week and water stress was imposed by withholding water from half of the pots for 20 days. Data were recorded on the relative water content (RWC), proline, sugar content and transcript...
Jun-Ying Liu, He-He Liu*, Jie Kou, Yang-Mei Zhao, Ji-Wen Wang, Liang Li and Xiao-Hui Du
... with an increase in the concentrations of immunoglobulin-G (IgG) and interleukin-12 (IL-12) in the serum of ducks at 1 day and 7 days after LPS injection (P<0.05). However, there were no apparent changes in organ index or the relative expression of TLR4 and IgM (P>0.05). Furthermore, correlation analysis showed a positive relationship between the mRNA expression of TLR4 and concentrations of TNF-&...
Sherin Reda Rouby
...ds that reflect the high concentrations of the virus. The results of the current study confirm the suitability of RP030 gene in differentiation of lumpy skin disease virus and sheep poxvirus in a single PCR assay without the necessity of post processing steps.
...
Shagufta Andleeb*1, Fariha Zahid1, Noor-ul-Ain1, Yousra Nisar1, Chaman Ara2
...mesticus). Different concentrations of permethrin (0, 5, 10 and 20ppm) were prepared in sterilized distilled water. These doses were injected into eggs on 3rd day while recovery of embryos was done on 7th day of incubation. Disrupted embryos with microcephly, hydrocephaly, short neck, micromelia, amelia, micrognathia, agnathia, cataract, ectopiacordis, omphalocoel, axis distortion, anencephaly, anophthalmia, microphthalmia, phocomelia...
Noor Fatima1, Muhammad Irfan2,*, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1
...evels like sulfuric acid concentrations (0.6%, 0.8%, 1% v/v), biomass loading (5%, 10%, 15% w/v), and residence time (4, 6 and 8h). After pretreatment process, cellulase production was achieved by solid substrate which was conducted in 250mL capacity Erlenmeyer flask in which incubation of Bacillus subtilis was carried out for 24 h of fermentation period at temperature of 50oC. Results showed that cellulase production was greatly affected by ...
Felicia NkechiEkeh*, Ifeanyi Oscar Ndimkaoha Aguzie, Joy Ihuoma Nzei, Chinenye Maria-Goretti Ohanu, Godwin Ikechukwu Ngwu and ChukwudiebubeUgolo
...wo extracts in different concentrations, a total of 63 sundried adult C. gariepinus of mean weight 111.47 ± 2.34g were randomized into three treatment groups A – C for each extract and a control D. The treatment groups were in triplicates with each containing three catfish. The groups contained fish infested with 20 larvae, 20 adults and eggs of D. maculatus. Groups A – C were exposed to 8, 12 and 15% ( w/w) of extract per kilo...
Gulsanga1, Zahin Anjum1, Sahib Alam2* and Farhat Shehzad1
...d that Cu, Zn, Pb and Ni concentrations in cow meat ranged from 7.27 to 14.81, 64.00 to 112.12, 3.30 to 6.25 and 0.73 to 2.53 mg/kg whereas the concentrations of these heavy metals in buffalo meat varied from 3.15 to 7.71, 72.88 to 88.47, 1.41 to 13.28 and 0.38 to 1.93 mg/kg, respectively. The average Na, K, Ca, Mg and P contents in cow’s and buffalo’s meat were found to be 54.75 and 67.45, 351.67 and 333.75, 4.6...
Huma Naz1,*, Sajid Abdullah2, Sidra Naz3, Sidra Abbas2, Wardah Hassan2, Shakeela Perveen2, Moazama Batool1, Laiba Shafique2
...each of the 19 different concentrations (5-90 mg/L) of Fe+Zn+Pb+Ni mixture to determine 96 h LC50 and lethal concentration. During acute toxicity test, behavioral changes and catalase activity in liver, kidney, gills, brain and muscle of the fish were also studied. The mean 96 h LC50 and lethal value for C. mrigala was determined as 47.56±0.04 and 93.89±0.07 mgL-1, respectively. The selected concentration o...
Farah Rauf Shakoori1,*, Anum Feroz1, Ayesha Gondal1,Sahar Akram2 and Tanzeela Riaz3
...olism and macromolecular concentrations of 4th and 6th instar larvae of a stored product pest, Trogoderma granarium. The LC50 values of λ-cyhalothrin for 4th and 6th instar larvae of Lahore population was 15.93 and 13.76ppm respectively, while 19.07 and 16.21ppm were for the 4th and 6th instar larvae of Gujranwala population, respectively. The resistance ratio RR (0.67 a...
Nashwa Mohamed Eid1, Al-Hussien Mohamed Dahshan1, El-Shayma El-Nahass2, Basma Shalaby3 and Ahmed Ali1*
...The influence of varying concentrations of both EOs was determined in vitro using the agar dilution test. Concentration as low as 0.25% and 0.125% of thyme and clove oils, respectively, were able to completely inhibit the growth of CP. An in vivo study was conducted in commercial broiler chickens, where, 2 groups of 10 day-old broilers were fed a balanced ration with addition of thyme (0.25%) or clove (0.125%). Birds were then challenged with CP (1×108 C...
Mohammed Al Bratty1, Hassan A. Alhazmi1, Somaya Jadoh Ogdi1, Jana Ahmad Otaif1, Abdul Jabbar Al-Rajab2, Mohammad Firoz Alam3 andSadique Akhtar Javed1,*
...ctrometry (ICP-OES). The concentrations of heavy metals in various chicken parts were recorded to be in the range of nd-0.01, 0.07-0.18, 1.08-3.95, 9.61-119.0, nd-0.16, 0.13-1.10, and 8.63-67.58 mg/kg for Cd, Cr, Cu, Fe, Ni, Pb and Zn respectively. Relatively higher metal concentrations were observed in bone, lungs and brain, which are generally not consumed by the local population. Meat, the most consumed part of the chicke...
Sukirno Sukirno1,2, Muhammad Tufail1,3,*, Khawaja Ghulam Rasool1, Said El Salamouny1, 4, Koko Dwi Sutanto1 and Abdulrahman Saad Aldawood1
... to determine the lethal concentrations of this virus against the first and second larval instar of S. exigua. The potency of ten plant extracts as UV absorbers were done by measuring the absorption spectra of the 0.5% extracts using UV spectrophotometer. Based on preliminary data, clove, green tea and henna extracts at 10% (v/v) concentration were added to the virus and tested under semi-field conditions on the cabbage plantations for 7 days. The preli...
Wasim Javaid1, Farid Asif Shaheen1*, Farah Naz2 and Muhammad Usman Raja2
...y using different fungal concentrations of 1×106, 1×107 and 1×108 spores/ml in stored chickpea grains. Highest mortality (90%) of PB was observed in 72 hours through application of 1×108spores/ml of M. anisopliae compared to B. bassiana with 60% mortality. On 24 hours of exposure, minimum mortality (4%) in PB was recorded in grains treated with 1×106 spores/ml of B. bassiana. In M. anisopliae, the concentr...
Muhammad Anwar Iqbal1, Habib Rehman2, Ali Hussain3,*, Faiza Jabeen4, Ijaz Ahmad2, Kamran Ashraf5, Muhammad Irshad Arshad6 and Omaima Khan7
...ineral levels and caecal concentrations of E. coli and Clostridium spp. in Japanese quail (Coturnix coturnix japonica). Japanese quail (one-day old; n = 1320) were taken and randomly separated into four groups (n = 320), having 8 replicates (n = 40) in each group. Corn-based poultry feed (basal diet) was given to control group birds (Group A) and the same feed mixed with three different concentrations of...
Madeeha Arshad1, Asmatullah1, Chaman Ara1, Shagufta Andleeb2 and Naveed Ahmad3,*
... mice fetuses. Different concentrations of the drug, 0.00, 4.10, 8.33 and 16.66 µg/g B.W. were administered orally to the dams on days 6-12 of gestation, and fetuses were recovered on day 18. Morphological observations of fetuses from 4.10, 8.33 and 16.66 µg/g B.W. dose groups showed abnormalities such as skin hemorrhages, microphthalmia, limb deformities, hygromas, kyphosis, curved and short tail and fluid filled abdominal cysts. Significant incre...
Kamran Khan1, Sarzamin Khan2, Nazir Ahmad Khan2,* and Naseer Ahmad2
...antify the effect of two concentrations and technological forms of flaxseed supplementation on growth performance, carcass yield and meat quality of rabbits. Sixty indigenous rabbits were divided randomly into five treatment groups. The control group was fed on standard concentrate basal diet, whereas in the treated groups, the basal diet was supplemented with low (3.5%; LCF) or high (7%; HCF) level of crushed flaxseed and low (1.5%; LFO) or high (3%; HFO) lev...
Fiaz Ahmad1, Mumtaz Hussain2, Ghulam Abbas1, Muhammad Jawad Asghar1 and Muhammad Rizwan3*
... to three different Pb2+ concentrations under two water stress conditions. Different morpho-physiological and yield traits were studied. Both varieties showed gradual reduction in morphological traits at increasing concentration of lead and water stress. Moreover, morphological traits were more severely affected by combined lead and water stress treatment (80 mg Pb2+ 50% FC) as compared to single lead (40 mg Pb2+ 100% FC and 80 mg Pb2+ 100% FC) and single wate...
Habib-ur-Rehman1*, Saima Mirza1*, Mansoor-ul-Hasan2, Qurban Ali3, Hafiz Abdullah Shakir4, Muhammad Yasir5
...th micropipette at three concentrations (5, 10 and15%) of each of the plant extract. The repellence was recorded after 24, 48 and 72 h of the treatments application. The findings of experimental trials presented significant mean repellency 79.15% of T. castaneum (with methanolic extract of C. paradise)followed by 76.21% (with R. communis extract)and 63.36% (in J.curcus extract) at 15% concentration, after exposure peri...
Farah Rauf Shakoori1,*, Tanzeela Riaz2, Uzma Ramzan1, Anum Feroz1 and Abdul Rauf Shakoori2,3,*
...olism and macromolecular concentrations of 4th and 6th instar larvae of a stored grain pest, Trogoderma granarium. The LC50 values of esfenvalerate for 4th and 6th instar larvae of Phosphine and deltamethrin-susceptible population was 34.29 and 28.05ppm, respectively, while 39.30 and 32.67ppm were for the 4th and 6th instar larvae of Phosphine and deltamethrin-tolerant populati...
Muhammad Zahid1, Naeem Iqbal1, Sohaib Muhammad2*, Summiya Faisal3, Wajid Mahboob3, Makhdoom Hussain4 and Zaheer ud din Khan2
... was supplied in various concentrations (0, 5, 10, 25 and 50 mM) as seed priming and foliar spray treatments at vegetative and reproductive stages. Plant growth and grain yields were reduced under drought. Two glucose treatments (10 mM and 50 mM) improved tillering and dry biomass as compared to other treatments. Foliar sprayed-glucose treated plants showed increasing trends in grain yield under drought. Physio-chemical attributes were also modulated by exogen...
Nevran Eylem Akman Gündüz1,* and Özgür Özcan2
...oration assay. Different concentrations (2, 5, 10, 50, 100, 200, 500 and 1000 mg/L) of GA3 were added to the synthetic diet and protein, lipid, carbohydrate and glycogen levels in the hemolymph were evaluated for the GA3 concentrations. The hemolymph protein level of the larvae increased significantly at 5, 10, 50 and 200 mg/L with respect to the control group. The lipid level of hemolymph fluctuated am...
Jam Nazeer Ahmad1,2,*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2, Sumaira Maqsood3, Ishita Ahuja4 and Atle M. Bones4
...ied by testing different concentrations (1 x 102 POB (Polyhedral occlusion bodies) mL-1- 1 x 1010 POB mL-1) from the occlusion bodies produced from NPV isolates against 2nd, 3rd, 4th and 5th instar larvae of S. litura. The rapid and sensitive polymerase chain reaction (PCR) technique was used for the molecular detection of NPV gene from native NPV diseased insect. Mu...
Ali Sher1*, Khalid Naveed1, Gulzar Ahmad2, Ayub Khan1, Muhammad Saeed1 and Shah Masaud1
... and 150 kg ha-1), three concentrations of Zn and Fe (i.e. 1, 2, 3 kg ha-1) each along with two controls i.e. no micro nutrients no water (control-I) and no micro nutrients + water (control-II). The N was soil applied and MN as foliar spray at booting stage. Days to anthesis, leaf area tiller-1, leaf area index, plant height, days to physiological maturity and biological yield were significantly increased by both N and Zn while the same parameters except days ...
Safina Naz1, Muhammad Akbar Anjum1, Saeed Akhtar2, Syed Atif Hasan Naqvi3* and Muhammad Asif Zulfiqar4
...sewage water had greater concentrations of these metals, which were higher than the safe limits recommended by WHO (1996). All the vegetables grown with tubewell water had significantly lower heavy metal contents, well below the critical limits. The concentration of Pb in tomato and cauliflower, Cd in tomato and spinach, and Fe in spinach irrigated with canal water were also detected beyond the safe limits. The study concludes that the continuous use of sewage...
Wajid Ali1,*, Asma Karim1, Muhammad Irfan2, Hafiz Abdullah Shakir3*, Ghulam Mustafa4, Zeeshan Shafique1, Ijaz Anwar1
...re exposed to sub lethal concentrations of deltamethrine. Fish were divided into 4 groups and each group contains six fish. Experimental groups viz. A, B and C were exposed to 25, 50 and 75% of LC50, respectively for four days. Blood samples for biochemical analysis (blood glucose, cholesterol and total protein) and gills for histology were taken after 96 hrs. After acute exposure to pesticide, significant changes were observed in serum bioch...
Soumble Zulfiqar1, Khuram Shehzad1, Sana Tahir1, Khalid A. Al-Ghanim2 and Abdul Rauf Shakoori1,2,3,*
...consequences at elevated concentrations. The bacterial cell utilizes a battery of copper detoxification and exclusion mechanisms of which multicopper oxidase (CueO) is an integral component. In addition to acting as copper regulatory elements, CueOs have been shown to possess laccase activity. In this study, we have cloned and over-expressed the CueO of locally isolated Klebsiella pneumoniae KW strain. The CueO protein was purified to homogeneity b...
Sabahat Noor*1, Shaukat Ali1, Hafeez-ur-Rahman1, Farhatullah2 and Ghulam Muhammad Ali1
...different levels of salt concentrations separately. Four parameters i.e. sugar contents, proline contents, chlorophyll contents and relative water contents were recorded and compared in transformed and non-transformed plants. It was observed that under drought and salt stresses, proline contents, sugar contents, chlorophyll contents and relative water contents (RWC) were significantly higher in transgenic plants as compared to non-transgenic plants. From obtai...
Ghazunfar Rashid1, Muhammad Avais1, Syed Saleem Ahmad1, Muhammad Hassan Mushtaq2, Rais Ahmed3,*, Mahboob Ali1, Muhammad Naveed-ul-Haque4, Mehtab Ahmad5, Mumtaz Ali Khan1 and Naimat Ullah Khan6
...i, Bajra and Sarson. The concentrations were lower in the afternoon in the leaves and in mature crops as compared to stem parts, immature plants, and in samples collected from plants during morning hours. The nitrate concentration was lower in samples collected from Ravi area, as compared to samples collected from villages of Pattoki and Okara. Blood samples were collected from animals feeding on above fodders; Spectrophotometry analysis of blood samples from ...
Sajid Ali Khan1, Mazhar Hussain Ranjha1, Azhar Abbas Khan2,*, Muhammad Sagheer1, Amjad Abbas1 and Zeshan Hassan2
... solvent. Four different concentrations 5%, 10%, 15% and 20% were prepared by diluting in acetone. The data regarding mortality, growth regulation and repellency of T. granarium was observed. Repellency of plant extracts was tested using area preference method while for the growth regulatory potential of plant extracts, the adults insects were released in wheat grains treated with different concentrations of plant ext...
Syed Atif Hasan Naqvi1*, Ummad-ud-Din Umar1, Ammarah Hasnain2, Ateeq ur Rehman1 and Rashida Perveen1
...n were tested at various concentrations under in vitro conditions by poison food and disk diffusion techniques while best effective seven were trialed in glass house and field experiments to determine their efficacy against Xanthomonas oryzae pv. oryzae. In vitro and in vivo studies under different conditions showed significant response of Mentha piperita, Azadirachta indica and Aloe vera either tested individually or in combination of two or more than two dec...
Muhammad Akram and Faheem Aftab
THIDIAZURON INDUCES IN VITRO BUD BREAK AND SHOOT DEVELOPMENT FROM NODAL EXPLANTS OF ORTHOTROPIC SHOOTS OF MAIDENHAIR TREE (GINKGO BILOBA L.)
...y, all other TDZ’s concentrations below 1 uM were also effective in bud breaking and development of shoots with green and vigorous growth. The frequency of bud break was significantly reduced with the increasing concentrations of TDZ. There were 2 phases of callus formation on the explant, i.e., before bud break and after the break. The frequency of callus induction was highest (100%) at 5uM TDZ before bud break along ...
Tanzeela Riaz*†, Farah Rauf Shakoori, Syed Shahid Ali
Toxicity of phosphine against tolerant and susceptible populations of Trogoderma granarium collected from Punjab, Pakistan
... to different sub lethal concentrations of phosphine for 20 hours. Based on LC50 data, Gujranwala population was found to be most resistant to phosphine whereas Sargodha population was found the least resistant population. Gujranwala population required 113.5, 128.8 and 168.1% more phosphine concentration in 4th
Hamda Azmat1, Waqas Ali2, Arshad Javid2, Ali Hussain2, Syed Makhdoom Hussain3, Zara Saeed1,Syed Shafiq Hussain Bukhari4
Chromium contamination in water, sediment and its bioaccumulation in Indian major carps in River Chenab, Pakistan
...tudy period, mmaximum Cr concentrations from upstream and downstream were recorded as 23.54± 3.43 mg/L and 21.12 ± 3.47 mg/L, respectively. Average metal concentration detected from sediment samples was 71.08±8.32 mg/L. Maximum Cr concentration 81.72±2.54 was recorded during February while minimum 61.24±1.23 mg/L during May. Among all the three fish species, maximum metal concentration was accumulated by Cirrhinus mrigala fol...
Muhammad Asghar1*, Mirza Muhammad Qadeer Baig1, Sanaullalh Chaudhary1 and Muhammad Anjum Ali2
...noconazole) with various concentrations of sprayable formulations of macronutrients (NPK) was conducted during Kharif 2012 to 14 at Adaptive Research Farm, Gujranwala, Pakistan. The experiment was conducted on a promising basmati rice cultivars Super basmati in RCBD with three repeats. The spray of difenoconazole at rate of 315 mlha-1 + 500gha-1 of NPK (20:20:20) significantly decreased incidence of BLS per leaf (9.31) and resulted in significant increase in 1...
Atiq-ur-Rehman1,2,*, Abida Latif1,*, Rukhsana Anwar1, Nasir Abbas1, Sajid Nawaz2 and Hamid Turab Mirza3
...ibitory assay. Different concentrations of fractions (20-100 µg/ml) were subjected to in vitro α-amylase inhibitory assay. Assessment of anti-hyperglycemic potential of the most active chloroform fraction exhibiting in vitro α-amylase inhibition was carried out in Wistar albino rat models. Changes in postprandial levels of blood glucose in rat models (normal and diabetic) were examined after starch load with or without chlorofor...
Muhammad Muddassir Ali1,2 and İbrahim Hakkı Ciğerci1,*
....15; 6±2) at both concentrations, respectively. All groups were significantly different (P<0.05) from the control group at both concentrations. A current study concluded that T. turcica had the genotoxic effects on the liver cancerous cell line and alcoholic extracts showed the more DNA damage on HepG2 cells.
...
Misbahullah, Shad Khan Khalil, Asim Muhammad*, Afza Tabassum and Shah Fahad
...rghum extract with three concentrations (1:3, 1:4, 1:5), weedicide (affinity) with three different levels (1/3, 1/ 2, 2/3 of the recommended dose) and 3 times of application (emergence,tillering,50% at emergence + fifty percent at tillering). Herbicide sole, weeded by hand and un weeded plots used for comparison. Results showing that concentration of sorghum and appliance point considerably increased days to heading, anthesis, physiological maturity, area of f...
Shagufta Nighat1, Muhammad Sajid Nadeem1,*, Syed Israr Shah1, Amber Khalid1, Tariq Mahmood2, Ayesha Aihetasham3 and Muhammad Asif4
... lead (Pb) and zinc (Zn) concentrations in bat tissues through atomic absorption spectrophotometer. Our findings showed that metals were more concentrated in the liver and kidneys, Zn and Pb showed high mean values as compared to other metals but these values were below the lethal levels. Within the regions and sexes no significant variations were found in metal concentrations; however within three bat species metal
Muhammad Ijaz1, Shahid Hussain Farooqi1, Rahmatullah2, Amjad Islam Aqib1, Sadaqat Ali3,*, Awais Ghaffar1, Ahmad Ali1 and Sehrish Saleem1
...copper, iron and lithium concentrations significantly (p < 0.05) decreased while the potassium concentration significantly (p < 0.05) increased in diarrheic foals. Hence, this can be concluded that diarrhea in foals have significant correlation with PCV, serum electrolytes, and trace and ultra-trace elements.
...
Mirza Imran Shahzad1, Hina Ashraf2,*, Muhammad Arshad3, Sabeeha Parveen4, Amna Aslam4, Nargis Naz4, Zahid Kamran1, Sumbul Gohar Khalid5, Sajid Hameed1, Muhammad Ashfaq6 and Muhammad Mukhtar7
...urther used in different concentrations and IC50 of each extract was calculated. The extract of Achyranthes aspera was found most effective with IC50 3.125μg and the extract of Oxystelma esculentum, was found least effective with IC50 60μg in the series. The current study has established the fact that Cholistani plants are rich source of anti-NDV agents and can be used in purified or in crude form.
...
Yan-Guo Han1, Xiao-Li Peng1, Kai Li1, Yu-He-Tian Zhao1, Xun-Ping Jiang2, Guang-Xin E1, Yong-Ju Zhao1, Jun-Hua Ye3, Li Xu3, Qin-Tao Zhao3 and Yong-Fu Huang1,*
...growth hormone and IGF-1 concentrations and average body weight than those in the control group (P < 0.05). Immunisation with ptCS/2SS-asd promoted the growth of rams similar to the effect of the simple co-injection of CpG and SS DNA vaccine. Thus, the fusion type of CpG adjuvant is an effective, simple, and low-cost method in enhancing the effect of SS DNA vaccine and the growth of ram lambs.
... 
